| 1 |  Elisa Balsamo (ITA) | Valcar - Travel & Service | 2:16:49 |
| 2 |  Lorena Wiebes (NED) | Parkhotel Valkenburg | mt |
| 3 | Marta Bastianelli (ITA) | Alé - Btc Ljubljana | mt |
| 4 | Chiara Consonni (ITA) | Valcar - Travel & Service | mt |
| 5 | Silvia Zanardi (ITA) | Be Pink | mt |
| 6 | Barbara Guarischi (ITA) | Movistar Team | mt |
| 7 |  Lisa Brennauer (GER) | Ceratizit - Wnt | mt |
| 8 |  Sandra Alonso Domínguez (ESP) | Casa Dorada | mt |
| 9 |  Jelena Eric (SRB) | Movistar Team | mt |
| 10 | Elisa Longo Borghini (ITA) | Trek - Segafredo | mt |
| 11 |  Alice Wood (Barnes) (GBR) | Canyon - Sram Racing | mt |
| 12 |  Sarah Roy (AUS) | Mitchelton - Scott | mt |
| 13 | Lucy Garner - van Der Haar (GBR) | Hitec Products - Birk Sport | mt |
| 14 | Minke Bakker (NED) | Doltcini - Van Eyck Sport | mt |
| 15 | Elizabeth Holden (GBR) | Bizkaia - Durango | mt |
| 16 |  Eugenia Bujak (SLO) | Alé - Btc Ljubljana | mt |
| 17 | Ursa Pintar (SLO) | Alé - Btc Ljubljana | mt |
| 18 | Maaike Boogaard (NED) | Alé - Btc Ljubljana | mt |
| 19 | Ellen Van Dijk (NED) | Trek - Segafredo | mt |
| 20 |  Christina Schweinberger (AUT) | Doltcini - Van Eyck Sport | mt |
| 21 |  Leah Kirchmann (CAN) | Sunweb | mt |
| 22 | Ilaria Sanguineti (ITA) | Valcar - Travel & Service | mt |
| 23 |  Audrey Cordon-Ragot (FRA) | Trek - Segafredo | mt |
| 24 | Maria Giulia Confalonieri (ITA) | Ceratizit - Wnt | 0:08 |
| 25 | Giorgia Catarzi (ITA) | Be Pink | 0:08 |
| 26 |  Georgia Williams (NZL) | Mitchelton - Scott | 0:08 |
| 27 |  Vita Heine (NOR) | Hitec Products - Birk Sport | 0:08 |
| 28 | Emilie Fortin (CAN) | Macogep - Tornatech - Specialized - Mazda | 0:08 |
| 29 |  Julie Norman Leth (DEN) | Ceratizit - Wnt | 0:08 |
| 30 | Katrine Aalerud (NOR) | Movistar Team | 0:08 |
| 31 | Rachel Neylan (AUS) | Casa Dorada | 0:08 |
| 32 | Simona Frapporti (ITA) | Be Pink | 0:08 |
| 33 | Hannah Ludwig (GER) | Canyon - Sram Racing | 0:08 |
| 34 | Marta Cavalli (ITA) | Valcar - Travel & Service | 0:08 |
| 35 | Janneke Ensing (NED) | Mitchelton - Scott | 0:08 |
| 36 | Jessica Roberts (GBR) | Mitchelton - Scott | 0:08 |
| 37 | Mieke Kröger (GER) | Hitec Products - Birk Sport | 0:08 |
| 38 | Marieke van Witzenburg-de Groot (NED) | Doltcini - Van Eyck Sport | 0:08 |
| 39 | Claire Steels (GBR) | Sopela Women's Team | 0:08 |
| 40 | Gabrielle Pilote-Fortin (CAN) | Massi - Tactic | 0:08 |
| 41 | Carolina Esteban Fernandez (ESP) | | 0:08 |
| 42 | Sara Martín Martín (ESP) | Sopela Women's Team | 0:08 |
| 43 | Mireia Trias Jordán (ESP) | Massi - Tactic | 0:08 |
| 44 |  Rotem Gafinovitz (ISR) | | 0:08 |
| 45 |  Eri Yonamine (JAP) | Alé - Btc Ljubljana | 0:08 |
| 46 | Laura Asencio (FRA) | Ceratizit - Wnt | 0:08 |
| 47 | Lara Vieceli (ITA) | Ceratizit - Wnt | 0:08 |
| 48 | Sarah Rijkes (AUT) | | 0:08 |
| 49 | Annemiek Van Vleuten (NED) | Mitchelton - Scott | 0:08 |
| 50 | Paula Díaz López (ESP) | | 0:08 |
| 51 | Mireia Benito Pellicer (ESP) | Massi - Tactic | 0:08 |
| 52 |  Nicole Hanselmann (SUI) | Doltcini - Van Eyck Sport | 0:08 |
| 53 | Belén López Morales (ESP) | Massi - Tactic | 0:08 |
| 54 | Liane Lippert (GER) | Sunweb | 0:08 |
| 55 |  Wilma Olausson (SWE) | Sunweb | 0:08 |
| 56 | Julia Soek (NED) | Sunweb | 0:08 |
| 57 | Vania Canvelli (ITA) | Be Pink | 0:08 |
| 58 | Camilla Alessio (ITA) | Be Pink | 0:08 |
| 59 | Christa Riffel (GER) | Canyon - Sram Racing | 0:08 |
| 60 |  Malgorzata Jasinska (POL) | Casa Dorada | 0:08 |
| 61 | Alicia González Blanco (ESP) | Movistar Team | 0:08 |
| 62 | Silvia Valsecchi (ITA) | Be Pink | 0:08 |
| 63 | Alba Teruel Ribes (ESP) | Movistar Team | 0:24 |
| 64 | Vittoria Guazzini (ITA) | Valcar - Travel & Service | 0:36 |
| 65 | Barbara Malcotti (ITA) | Valcar - Travel & Service | 0:47 |
| 66 | Gloria Rodríguez Sanchez (ESP) | Movistar Team | 1:12 |
| 67 | Laura Tenorio Herrero (ESP) | | 1:15 |
| DNF | Letizia Paternoster (ITA) | Trek - Segafredo | - |
| DNF | Anna Trevisi (ITA) | Alé - Btc Ljubljana | - |
| DNF | Emma Ortiz Bonilla (ESP) | Sopela Women's Team | - |
| DNF | Jessica Allen (AUS) | Mitchelton - Scott | - |
| DNF | Alessia Vigilia (ITA) | Casa Dorada | - |
| DNF | Martina Moreno Monteys (ESP) | | - |
| DNF | Ella Harris (NZL) | Canyon - Sram Racing | - |
| DNF | Lydia Iglesias Bares (ESP) | Casa Dorada | - |
| DNF | Patricia Ortega Ruiz (ESP) | Massi - Tactic | - |
| DNF |  Alexis Ryan (USA) | Canyon - Sram Racing | - |
| DNF | Ariana Gilabert Vilaplana (ESP) | Bizkaia - Durango | - |
| DNF | Susana Perez Conejero (ESP) | | - |
Image © Amaury Sport Organisation (ASO). Source : Site officiel du Madrid Challenge by La Vuelta.
Doltcini - Van Eyck Sport