| 1 |  Flávia Oliveira (BRA) | Lensworld - Zannata - Etixx | 23:21 |
| 2 |  Arlenis Sierra Cañadilla (CUB) | | 0:13 |
| 3 |  Ingrid Drexel Clouthier (MEX) | Astana Women's | 0:20 |
| 4 |  Blanca Liliana Moreno Canchon (COL) | | 0:48 |
| 5 |  Paula Cristina Herrera Hernandez (CRC) | | 1:14 |
| 6 | Sofia Arreola Navarro (MEX) | Twenty16 - Ridebiker | 1:28 |
| 7 | Carolina Rodriguez Gutierrez (MEX) | Astana Women's | 1:54 |
| 8 | Milagro Consuelo Mena Solano (CRC) | | 1:57 |
| 9 | Estefanía Herrera Marin (COL) | | 2:00 |
| 10 | Katherin Steisy Montoya Zuluaga (COL) | | 2:22 |
| 11 | Yossiana Quintero (COL) | | 2:28 |
| 12 | Maria José Vargas Barrientos (CRC) | | 2:29 |
| 13 | Edith Guillen Chavarria (CRC) | | 2:33 |
| 14 |  Evelyn Yesenia García Marroquin (ESA) | | 2:51 |
| 15 | Mayra del Rocio Rocha Guerrero (MEX) | | 3:01 |
| 16 |  Danielys del Valle García Buitriag (VEN) | | 3:01 |
| 17 | Susan Camacho Fallas (CRC) | | 3:02 |
| 18 |  Aranza Valentina Villalon Sanchez (CHI) | Weber - Shimano Ladies Power | 3:04 |
| 19 | Luiza Daniela Hernández Torres (COL) | | 3:08 |
| 20 | Stephanie Nathalie Subercaseaux Vergara (CHI) | | 3:22 |
| 21 | Erika Haydee Varela Huerta (MEX) | | 3:28 |
| 22 | Jennifer Mariana Cesar Salazar (VEN) | | 3:30 |
| 23 | Marcela Rubiano Garces (CRC) | | 3:38 |
| 24 | Gigliolla Monichi (CHI) | | 3:43 |
| 25 | Hazel Sancho Gonzalez (CRC) | | 4:08 |
| 26 |  Florida Isabel De León Gonzalez (GUA) | | 4:20 |
| 27 | Rosimber Montaña (VEN) | | 4:21 |
| 28 | Karla Vallejos Puchi (CHI) | | 4:40 |
| 29 | Zuralmy Coromoto Rivas Molina (VEN) | | 4:42 |
| 30 | Lisbeth Alvarado (CRC) | | 4:43 |
| 31 | Natalia Navarro Cerdas (CRC) | | 5:05 |
| 32 | Ingrid Ramirez (CRC) | | 5:06 |
| 33 | Ingrid Mayeli Porras Ochoa (VEN) | | 5:11 |
| 34 | Luisa Fernanda Naranjo Henao (COL) | | 5:12 |
| 35 | Daniela Martinez (CRC) | | 5:16 |
| 36 | Eunice Rojas (CRC) | | 5:19 |
| 37 | Javiera Reyes (CHI) | | 5:35 |
| 38 | Sofía Chaverry (CRC) | | 5:41 |
| 39 | Gabriela Arias (CRC) | | 5:48 |
| 40 | Diana Solano Rojas (CRC) | | 5:48 |
| 41 | Katherine Herrera Fernandez (CRC) | | 5:48 |
| 42 | Yulieth Arroyo (CRC) | | 5:50 |
| 43 | Maria Sofia Morales Fallas (CRC) | | 5:51 |
| 44 | Caterina Tellini (CRC) | | 6:30 |
| 45 | Sharon Montenegro (CRC) | | 6:35 |
| 46 |  Yineth Kellyam Cubilla (PAN) | | 6:40 |
| 47 | Kristie Kibler (CRC) | | 6:45 |
| 48 | Cristina Cortes (CRC) | | 6:50 |
| 49 | Ana Teresa Casas Bonilla (MEX) | | 6:56 |
| 50 | Kennia Damaris Vargas Umaña (CRC) | | 7:01 |
| 51 | Tania Pamela Carrera Mora (CRC) | | 7:02 |
| 52 |  Angie Gómez (HON) | | 7:10 |
| 53 | Sofia Howell (CRC) | | 7:13 |
| 54 | María Morales (GUA) | | 7:18 |
| 55 | Constanza Victoria Paredes Martinez (CHI) | | 7:20 |
| 56 | Wendy Ducreux (PAN) | | 7:22 |
| 57 | Jahzeel Blanco (HON) | | 7:23 |
| 58 | Wilmarys del Valle Moreno Duran (VEN) | | 7:26 |
| 59 | Jetty Chevez (CRC) | | 7:32 |
| 60 | Laura Lopez Torres (CRC) | | 7:45 |
| 61 | Diana Fernandez (CRC) | | 7:51 |
| 62 | Mauren Solano (CRC) | | 7:54 |
| 63 | Daniela Salcedo (PAN) | | 7:56 |
| 64 | Melissa Vargas (CRC) | | 8:07 |
| 65 | Tatiana Hidalgo (CRC) | | 8:11 |
| 66 | Alyson Chévez (CRC) | | 8:16 |
| 67 | Natalia Rodriguez (CRC) | | 8:16 |
| 68 | Maria José Segura Quesada (CRC) | | 8:48 |
| 69 | Angie Fernanda Oviedo Melendez (CRC) | | 8:52 |
| 70 | Cindy Tencio (CRC) | | 9:14 |
| 71 | Ana Cristina Marchena (CRC) | | 9:18 |
| 72 | Ana Victoria González Carrera (PAN) | | 10:13 |
| 73 | Karla González (CRC) | | 10:14 |
| 74 | Dora Elizondo (CRC) | | 10:14 |
| 75 | Gabriela Castillo (CRC) | | 10:26 |
| 76 | Rachida Jaikel (CRC) | | 10:31 |
| 77 | Stefanny Guerra (CRC) | | 10:35 |
| 78 | Nathalie Ulate (CRC) | | 10:40 |
| 79 | Saylyn Mayo (CRC) | | 10:55 |
| 80 | Daniela Ruiz (CRC) | | 11:29 |
| 81 | Johanna Jimenez Rojas (CRC) | | 11:45 |
| 82 | Lissette Carrillo (CRC) | | 12:04 |
| 83 | Cristina Diaz (CRC) | | 12:30 |
| 84 | Elizabeth Blanco (CRC) | | 12:55 |
| 85 | Wendy Arias (CRC) | | 14:08 |
| 86 | Roxana Maria Alvarado Lopez (CRC) | | 21:52 |
| 87 | Emma Jorge (PAN) | | |
